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methanol compatible syringe filter  (Sartorius AG)
96
Sartorius AG methanol compatible syringe filter
(a) Map of Turkey showing the coastal sampling stations (Triangle 1: Kocaeli, Marmara Sea coast; Triangle 2: Trabzon, Black Sea coast). (b) Collected seafood samples: European anchovy ( Engraulis encrasicolus ) and mussel ( Mytilus galloprovincialis ). (c) Sample mincing and blending. (d) Homogenization in methanol/water (1:1, v/v). (e) Centrifugation at 4000 rpm for 20 min. (f) Filtration of the supernatant using a 0.22 <t>μm</t> <t>methanol‐compatible</t> filter. (g) Storage of extracted samples in sterile tubes at 4°C. Each extraction step was conducted under sterile conditions. All seafood extracts were prepared using a standardized protocol involving homogenization in 1:1 methanol/water (v/v), centrifugation, filtration through 0.22 μm membrane filters, and storage at 4°C. The filtered extracts were later applied to the biosensor surface and incubated for 30 min. Each extraction was performed in triplicate to ensure reproducibility and consistency in biosensor response. Created in BioRender. Avci, H. (2025) https://BioRender.com/xfc9ssj .
Methanol Compatible Syringe Filter, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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methanol compatible syringe filter - by Bioz Stars, 2026-05
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methanol free formaldehyde  (Thermo Fisher)
99
Thermo Fisher methanol free formaldehyde
(a) Map of Turkey showing the coastal sampling stations (Triangle 1: Kocaeli, Marmara Sea coast; Triangle 2: Trabzon, Black Sea coast). (b) Collected seafood samples: European anchovy ( Engraulis encrasicolus ) and mussel ( Mytilus galloprovincialis ). (c) Sample mincing and blending. (d) Homogenization in methanol/water (1:1, v/v). (e) Centrifugation at 4000 rpm for 20 min. (f) Filtration of the supernatant using a 0.22 <t>μm</t> <t>methanol‐compatible</t> filter. (g) Storage of extracted samples in sterile tubes at 4°C. Each extraction step was conducted under sterile conditions. All seafood extracts were prepared using a standardized protocol involving homogenization in 1:1 methanol/water (v/v), centrifugation, filtration through 0.22 μm membrane filters, and storage at 4°C. The filtered extracts were later applied to the biosensor surface and incubated for 30 min. Each extraction was performed in triplicate to ensure reproducibility and consistency in biosensor response. Created in BioRender. Avci, H. (2025) https://BioRender.com/xfc9ssj .
Methanol Free Formaldehyde, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pre cooled methanol  (Fisher Scientific)
86
Fisher Scientific pre cooled methanol
(a) Map of Turkey showing the coastal sampling stations (Triangle 1: Kocaeli, Marmara Sea coast; Triangle 2: Trabzon, Black Sea coast). (b) Collected seafood samples: European anchovy ( Engraulis encrasicolus ) and mussel ( Mytilus galloprovincialis ). (c) Sample mincing and blending. (d) Homogenization in methanol/water (1:1, v/v). (e) Centrifugation at 4000 rpm for 20 min. (f) Filtration of the supernatant using a 0.22 <t>μm</t> <t>methanol‐compatible</t> filter. (g) Storage of extracted samples in sterile tubes at 4°C. Each extraction step was conducted under sterile conditions. All seafood extracts were prepared using a standardized protocol involving homogenization in 1:1 methanol/water (v/v), centrifugation, filtration through 0.22 μm membrane filters, and storage at 4°C. The filtered extracts were later applied to the biosensor surface and incubated for 30 min. Each extraction was performed in triplicate to ensure reproducibility and consistency in biosensor response. Created in BioRender. Avci, H. (2025) https://BioRender.com/xfc9ssj .
Pre Cooled Methanol, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pre cooled methanol/product/Fisher Scientific
Average 86 stars, based on 1 article reviews
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acetonitrile methanol water mixture  (Valiant Co Ltd)
94
Valiant Co Ltd acetonitrile methanol water mixture
(a) Map of Turkey showing the coastal sampling stations (Triangle 1: Kocaeli, Marmara Sea coast; Triangle 2: Trabzon, Black Sea coast). (b) Collected seafood samples: European anchovy ( Engraulis encrasicolus ) and mussel ( Mytilus galloprovincialis ). (c) Sample mincing and blending. (d) Homogenization in methanol/water (1:1, v/v). (e) Centrifugation at 4000 rpm for 20 min. (f) Filtration of the supernatant using a 0.22 <t>μm</t> <t>methanol‐compatible</t> filter. (g) Storage of extracted samples in sterile tubes at 4°C. Each extraction step was conducted under sterile conditions. All seafood extracts were prepared using a standardized protocol involving homogenization in 1:1 methanol/water (v/v), centrifugation, filtration through 0.22 μm membrane filters, and storage at 4°C. The filtered extracts were later applied to the biosensor surface and incubated for 30 min. Each extraction was performed in triplicate to ensure reproducibility and consistency in biosensor response. Created in BioRender. Avci, H. (2025) https://BioRender.com/xfc9ssj .
Acetonitrile Methanol Water Mixture, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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acetonitrile methanol water mixture - by Bioz Stars, 2026-05
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ice cold methanol acetonitrile water  (Valiant Co Ltd)
94
Valiant Co Ltd ice cold methanol acetonitrile water
(a) Map of Turkey showing the coastal sampling stations (Triangle 1: Kocaeli, Marmara Sea coast; Triangle 2: Trabzon, Black Sea coast). (b) Collected seafood samples: European anchovy ( Engraulis encrasicolus ) and mussel ( Mytilus galloprovincialis ). (c) Sample mincing and blending. (d) Homogenization in methanol/water (1:1, v/v). (e) Centrifugation at 4000 rpm for 20 min. (f) Filtration of the supernatant using a 0.22 <t>μm</t> <t>methanol‐compatible</t> filter. (g) Storage of extracted samples in sterile tubes at 4°C. Each extraction step was conducted under sterile conditions. All seafood extracts were prepared using a standardized protocol involving homogenization in 1:1 methanol/water (v/v), centrifugation, filtration through 0.22 μm membrane filters, and storage at 4°C. The filtered extracts were later applied to the biosensor surface and incubated for 30 min. Each extraction was performed in triplicate to ensure reproducibility and consistency in biosensor response. Created in BioRender. Avci, H. (2025) https://BioRender.com/xfc9ssj .
Ice Cold Methanol Acetonitrile Water, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ice cold methanol acetonitrile water/product/Valiant Co Ltd
Average 94 stars, based on 1 article reviews
ice cold methanol acetonitrile water - by Bioz Stars, 2026-05
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methanol hplc grade  (Carlo Erba Reagents)
86
Carlo Erba Reagents methanol hplc grade
(a) Map of Turkey showing the coastal sampling stations (Triangle 1: Kocaeli, Marmara Sea coast; Triangle 2: Trabzon, Black Sea coast). (b) Collected seafood samples: European anchovy ( Engraulis encrasicolus ) and mussel ( Mytilus galloprovincialis ). (c) Sample mincing and blending. (d) Homogenization in methanol/water (1:1, v/v). (e) Centrifugation at 4000 rpm for 20 min. (f) Filtration of the supernatant using a 0.22 <t>μm</t> <t>methanol‐compatible</t> filter. (g) Storage of extracted samples in sterile tubes at 4°C. Each extraction step was conducted under sterile conditions. All seafood extracts were prepared using a standardized protocol involving homogenization in 1:1 methanol/water (v/v), centrifugation, filtration through 0.22 μm membrane filters, and storage at 4°C. The filtered extracts were later applied to the biosensor surface and incubated for 30 min. Each extraction was performed in triplicate to ensure reproducibility and consistency in biosensor response. Created in BioRender. Avci, H. (2025) https://BioRender.com/xfc9ssj .
Methanol Hplc Grade, supplied by Carlo Erba Reagents, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/methanol hplc grade/product/Carlo Erba Reagents
Average 86 stars, based on 1 article reviews
methanol hplc grade - by Bioz Stars, 2026-05
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methanol  (Shanghai Aladdin Bio-Chem)
86
Shanghai Aladdin Bio-Chem methanol
(a) Map of Turkey showing the coastal sampling stations (Triangle 1: Kocaeli, Marmara Sea coast; Triangle 2: Trabzon, Black Sea coast). (b) Collected seafood samples: European anchovy ( Engraulis encrasicolus ) and mussel ( Mytilus galloprovincialis ). (c) Sample mincing and blending. (d) Homogenization in methanol/water (1:1, v/v). (e) Centrifugation at 4000 rpm for 20 min. (f) Filtration of the supernatant using a 0.22 <t>μm</t> <t>methanol‐compatible</t> filter. (g) Storage of extracted samples in sterile tubes at 4°C. Each extraction step was conducted under sterile conditions. All seafood extracts were prepared using a standardized protocol involving homogenization in 1:1 methanol/water (v/v), centrifugation, filtration through 0.22 μm membrane filters, and storage at 4°C. The filtered extracts were later applied to the biosensor surface and incubated for 30 min. Each extraction was performed in triplicate to ensure reproducibility and consistency in biosensor response. Created in BioRender. Avci, H. (2025) https://BioRender.com/xfc9ssj .
Methanol, supplied by Shanghai Aladdin Bio-Chem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
methanol - by Bioz Stars, 2026-05
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lc ms grade methanol  (Fisher Scientific)
86
Fisher Scientific lc ms grade methanol
(a) Map of Turkey showing the coastal sampling stations (Triangle 1: Kocaeli, Marmara Sea coast; Triangle 2: Trabzon, Black Sea coast). (b) Collected seafood samples: European anchovy ( Engraulis encrasicolus ) and mussel ( Mytilus galloprovincialis ). (c) Sample mincing and blending. (d) Homogenization in methanol/water (1:1, v/v). (e) Centrifugation at 4000 rpm for 20 min. (f) Filtration of the supernatant using a 0.22 <t>μm</t> <t>methanol‐compatible</t> filter. (g) Storage of extracted samples in sterile tubes at 4°C. Each extraction step was conducted under sterile conditions. All seafood extracts were prepared using a standardized protocol involving homogenization in 1:1 methanol/water (v/v), centrifugation, filtration through 0.22 μm membrane filters, and storage at 4°C. The filtered extracts were later applied to the biosensor surface and incubated for 30 min. Each extraction was performed in triplicate to ensure reproducibility and consistency in biosensor response. Created in BioRender. Avci, H. (2025) https://BioRender.com/xfc9ssj .
Lc Ms Grade Methanol, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n0446s methanol  (New England Biolabs)
96
New England Biolabs n0446s methanol
(a) Map of Turkey showing the coastal sampling stations (Triangle 1: Kocaeli, Marmara Sea coast; Triangle 2: Trabzon, Black Sea coast). (b) Collected seafood samples: European anchovy ( Engraulis encrasicolus ) and mussel ( Mytilus galloprovincialis ). (c) Sample mincing and blending. (d) Homogenization in methanol/water (1:1, v/v). (e) Centrifugation at 4000 rpm for 20 min. (f) Filtration of the supernatant using a 0.22 <t>μm</t> <t>methanol‐compatible</t> filter. (g) Storage of extracted samples in sterile tubes at 4°C. Each extraction step was conducted under sterile conditions. All seafood extracts were prepared using a standardized protocol involving homogenization in 1:1 methanol/water (v/v), centrifugation, filtration through 0.22 μm membrane filters, and storage at 4°C. The filtered extracts were later applied to the biosensor surface and incubated for 30 min. Each extraction was performed in triplicate to ensure reproducibility and consistency in biosensor response. Created in BioRender. Avci, H. (2025) https://BioRender.com/xfc9ssj .
N0446s Methanol, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(a) Map of Turkey showing the coastal sampling stations (Triangle 1: Kocaeli, Marmara Sea coast; Triangle 2: Trabzon, Black Sea coast). (b) Collected seafood samples: European anchovy ( Engraulis encrasicolus ) and mussel ( Mytilus galloprovincialis ). (c) Sample mincing and blending. (d) Homogenization in methanol/water (1:1, v/v). (e) Centrifugation at 4000 rpm for 20 min. (f) Filtration of the supernatant using a 0.22 μm methanol‐compatible filter. (g) Storage of extracted samples in sterile tubes at 4°C. Each extraction step was conducted under sterile conditions. All seafood extracts were prepared using a standardized protocol involving homogenization in 1:1 methanol/water (v/v), centrifugation, filtration through 0.22 μm membrane filters, and storage at 4°C. The filtered extracts were later applied to the biosensor surface and incubated for 30 min. Each extraction was performed in triplicate to ensure reproducibility and consistency in biosensor response. Created in BioRender. Avci, H. (2025) https://BioRender.com/xfc9ssj .

Journal: Food Science & Nutrition

Article Title: Development of a High‐Sensitivity Electrochemical Biosensor for Domoic Acid and Its Cellular Impact on Human Stem and Neuron‐Like Cells

doi: 10.1002/fsn3.71799

Figure Lengend Snippet: (a) Map of Turkey showing the coastal sampling stations (Triangle 1: Kocaeli, Marmara Sea coast; Triangle 2: Trabzon, Black Sea coast). (b) Collected seafood samples: European anchovy ( Engraulis encrasicolus ) and mussel ( Mytilus galloprovincialis ). (c) Sample mincing and blending. (d) Homogenization in methanol/water (1:1, v/v). (e) Centrifugation at 4000 rpm for 20 min. (f) Filtration of the supernatant using a 0.22 μm methanol‐compatible filter. (g) Storage of extracted samples in sterile tubes at 4°C. Each extraction step was conducted under sterile conditions. All seafood extracts were prepared using a standardized protocol involving homogenization in 1:1 methanol/water (v/v), centrifugation, filtration through 0.22 μm membrane filters, and storage at 4°C. The filtered extracts were later applied to the biosensor surface and incubated for 30 min. Each extraction was performed in triplicate to ensure reproducibility and consistency in biosensor response. Created in BioRender. Avci, H. (2025) https://BioRender.com/xfc9ssj .

Article Snippet: The supernatant was filtered through a 0.22 μm methanol‐compatible syringe filter (Minisart RC 25, Sartorius Stedim Biotech GmbH, Göttingen, Germany) and stored in sterile tubes at 4°C until further analysis.

Techniques: Sampling, Homogenization, Centrifugation, Filtration, Sterility, Extraction, Membrane, Incubation